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转录因子缓冲液试剂盒

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产品名称: 转录因子缓冲液试剂盒
产品型号: 562574
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简单介绍

Store undiluted at 4°C. Keep stock buffers and 1x working solutions at 2-8°C. After opening the BD Pharmingen™ Transcription Factor Buffer Set, use it within six months. If stored without opening, then use before the expiration date indicated on the bottle labels. Avoid microbial contamination of reagents as incorrect results may occur. Handle TF Diluent Buffer (Component 51-9008101) in a sterile


转录因子缓冲液试剂盒  的详细介绍

DESCRIPTION

The BD Pharmingen™ Transcription Factor Buffer Set is optimized for fixing and permeabilizing cells prior to immunofluorescent staining and flow cytometric analysis of cells that express specific intracytoplasmic and intranuclear proteins. The BD Pharmingen™ Transcription Factor Buffer Set was designed to improve ease-of-use and minimize processing time, to reduce nonspecific staining, to increase the resolution of positively stained cells and to significantly reduce cell loss during fixation, permeabilization and staining procedures. Flow cytometric detection of the many proteins known to be expressed within various intracellular compartments, especially transcription factors, is improved with BD Pharmingen™ Transcription Factor Buffer Set use. This buffer system has been found useful for fixing and permeabilizing a variety of cell types from diverse human and mouse tissues. The buffer system is flexible in supporting multiwell-plate high-throughput and bulk sample analyses and applications that require overnight sample storage. The buffer system has minimal impact on the light-scatter and autofluorescence characteristics of processed cells resulting in characteristics similar to those observed for freshly prepared, highly viable primary cells. In many cases the buffer system was found to be compatible with the immunofluorescent staining of cell-surface antigens both before and after cellular fixation and permeabilization. The buffer system is also compatible with many tandem fluorochromes.


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